Fragile X mental retardation protein interacts with the RNA-binding protein Caprin1 in neuronal RiboNucleoProtein complexes [corrected].

TitleFragile X mental retardation protein interacts with the RNA-binding protein Caprin1 in neuronal RiboNucleoProtein complexes [corrected].
Publication TypeJournal Article
Year of Publication2012
AuthorsFatimy REl, Tremblay S, Dury AY, Solomon S, De Koninck P, Schrader JW, Khandjian EW
JournalPLoS One
Volume7
Issue6
Paginatione39338
Date Published2012
ISSN1932-6203
KeywordsAmino Acid Motifs, Animals, Antibodies, Monoclonal, Base Sequence, Brain, Calcium-Calmodulin-Dependent Protein Kinase Type 2, Cell Cycle Proteins, DNA Primers, Fragile X Mental Retardation Protein, HeLa Cells, Humans, Immunoprecipitation, Mice, Mice, Inbred C57BL, Mice, Knockout, Microtubule-Associated Proteins, Molecular Sequence Data, Neurons, NIH 3T3 Cells, Polyribosomes, Protein Binding, Protein Biosynthesis, Ribonucleoproteins, RNA-Binding Proteins
Abstract

Fragile X syndrome is caused by the absence of the Fragile X Mental Retardation Protein (FMRP), an RNA-binding protein. FMRP is associated with messenger RiboNucleoParticles (mRNPs) present in polyribosomes and its absence in neurons leads to alteration in synaptic plasticity as a result of translation regulation defects. The molecular mechanisms by which FMRP plays a role in translation regulation remain elusive. Using immunoprecipitation approaches with monoclonal Ab7G1-1 and a new generation of chicken antibodies, we identified Caprin1 as a novel FMRP-cellular partner. In vivo and in vitro evidence show that Caprin1 interacts with FMRP at the level of the translation machinery as well as in trafficking neuronal granules. As an RNA-binding protein, Caprin1 has in common with FMRP at least two RNA targets that have been identified as CaMKIIα and Map1b mRNAs. In view of the new concept that FMRP species bind to RNA regardless of known structural motifs, we propose that protein interactors might modulate FMRP functions.

DOI10.1371/journal.pone.0039338
Alternate JournalPLoS ONE
PubMed ID22737234
PubMed Central IDPMC3380850
Grant List / / Canadian Institutes of Health Research / Canada

Funding

Our research endeavors are made possible by the following agencies:

Canadian Institutes of Health Research - Instituts de recherche en santé du Canada Fonds de recherche du Québec – Nature et technologies (FRQNT)Fonds de la recherche en santé du Québec NARSAD Natural Sciences and Engineering Research Council of Canada (NSERC) - Conseil de recherche en sciences naturelles et en génie du Canada (CRSNG)innovation.caHuman Frontier Science Program