Sensitivity of CaM kinase II to the frequency of Ca2+ oscillations.

TitleSensitivity of CaM kinase II to the frequency of Ca2+ oscillations.
Publication TypeJournal Article
Year of Publication1998
AuthorsDe Koninck P, Schulman H
JournalScience
Volume279
Issue5348
Pagination227-30
Date Published1998 Jan 9
ISSN0036-8075
KeywordsAnimals, Base Sequence, Calcium, Calcium-Calmodulin-Dependent Protein Kinase Type 2, Calcium-Calmodulin-Dependent Protein Kinases, Calmodulin, Cercopithecus aethiops, COS Cells, Enzyme Activation, Enzymes, Immobilized, Molecular Sequence Data, Neuronal Plasticity, Phosphorylation, Phosphothreonine, Polyvinyl Chloride, Recombinant Proteins, Signal Transduction
Abstract

The transduction of many cellular stimuli results in oscillations in the intracellular concentration of calcium ions (Ca2+). Although information is thought to be encoded in the frequency of such oscillations, no frequency decoder has been identified. Rapid superfusion of immobilized Ca2+- and calmodulin-dependent protein kinase II (CaM kinase II) in vitro showed that the enzyme can decode the frequency of Ca2+ spikes into distinct amounts of kinase activity. The frequency response of CaM kinase II was modulated by several factors, including the amplitude and duration of individual spikes as well as the subunit composition and previous state of activation of the kinase. These features should provide specificity in the activation of this multifunctional enzyme by distinct cellular stimuli and may underlie its pivotal role in activity-dependent forms of synaptic plasticity.

Alternate JournalScience
PubMed ID9422695
Grant ListGM30179 / GM / NIGMS NIH HHS / United States
GM40600 / GM / NIGMS NIH HHS / United States

Funding

Our research endeavors are made possible by the following agencies:

Canadian Institutes of Health Research - Instituts de recherche en santé du Canada Fonds de recherche du Québec – Nature et technologies (FRQNT)Fonds de la recherche en santé du Québec NARSAD Natural Sciences and Engineering Research Council of Canada (NSERC) - Conseil de recherche en sciences naturelles et en génie du Canada (CRSNG)innovation.caHuman Frontier Science Program