Translocation of CaMKII to dendritic microtubules supports the plasticity of local synapses.

TitleTranslocation of CaMKII to dendritic microtubules supports the plasticity of local synapses.
Publication TypeJournal Article
Year of Publication2012
AuthorsLemieux M, Labrecque S, Tardif C, Labrie-Dion É, Lebel E, De Koninck P
JournalJ Cell Biol
Volume198
Issue6
Pagination1055-73
Date Published2012 Sep 17
ISSN1540-8140
Keywordsalpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid, Animals, Calcium, Calcium-Calmodulin-Dependent Protein Kinase Type 2, Cells, Cultured, Dendrites, Glutamic Acid, Glycine, Hippocampus, Microtubules, Neuronal Plasticity, Neurons, Phosphorylation, Protein Transport, Rats, Receptors, AMPA, Spine, Synapses
Abstract

The processing of excitatory synaptic inputs involves compartmentalized dendritic Ca(2+) oscillations. The downstream signaling evoked by these local Ca(2+) transients and their impact on local synaptic development and remodeling are unknown. Ca(2+)/calmodulin-dependent protein kinase II (CaMKII) is an important decoder of Ca(2+) signals and mediator of synaptic plasticity. In addition to its known accumulation at spines, we observed with live imaging the dynamic recruitment of CaMKII to dendritic subdomains adjacent to activated synapses in cultured hippocampal neurons. This localized and transient enrichment of CaMKII to dendritic sites coincided spatially and temporally with dendritic Ca(2+) transients. We show that it involved an interaction with microtubular elements, required activation of the kinase, and led to localized dendritic CaMKII autophosphorylation. This process was accompanied by the adjacent remodeling of spines and synaptic AMPA receptor insertion. Replacement of endogenous CaMKII with a mutant that cannot translocate within dendrites lessened this activity-dependent synaptic plasticity. Thus, CaMKII could decode compartmental dendritic Ca(2+) transients to support remodeling of local synapses.

DOI10.1083/jcb.201202058
Alternate JournalJ. Cell Biol.
PubMed ID22965911
PubMed Central IDPMC3444784
Grant List / / Canadian Institutes of Health Research / Canada

Funding

Our research endeavors are made possible by the following agencies:

Canadian Institutes of Health Research - Instituts de recherche en santé du Canada Fonds de recherche du Québec – Nature et technologies (FRQNT)Fonds de la recherche en santé du Québec NARSAD Natural Sciences and Engineering Research Council of Canada (NSERC) - Conseil de recherche en sciences naturelles et en génie du Canada (CRSNG)innovation.caHuman Frontier Science Program