Wide-field multiphoton imaging of cellular dynamics in thick tissue by temporal focusing and patterned illumination.

TitleWide-field multiphoton imaging of cellular dynamics in thick tissue by temporal focusing and patterned illumination.
Publication TypeJournal Article
Year of Publication2011
AuthorsTherrien OD, Aubé B, Pagès S, De Koninck P, Côté D
JournalBiomed Opt Express
Volume2
Issue3
Pagination696-704
Date Published2011
ISSN2156-7085
Abstract

Wide-field temporal focusing is a novel technique that provides optical sectioning for imaging without the need for beam scanning. However, illuminating over large areas greatly reduces the photon density which limits the technique applicability to small regions, precluding functional imaging of cellular networks. Here we present a strategy that combines beam shaping and temporal focusing of amplified pulses (>1 µJ/pulse) for fast imaging of cells from the central nervous system in acute slices. Multiphoton video-rate imaging over total areas as wide as 4800 µm(2) with an optical sectioning under 10 µm at 800 nm is achieved with our setup, leading to imaging of calcium dynamics of multiple cells simultaneously in thick tissue.

DOI10.1364/BOE.2.000696
Alternate JournalBiomed Opt Express
PubMed ID21412473
PubMed Central IDPMC3047373

Funding

Our research endeavors are made possible by the following agencies:

Canadian Institutes of Health Research - Instituts de recherche en santé du Canada Fonds de recherche du Québec – Nature et technologies (FRQNT)Fonds de la recherche en santé du Québec NARSAD Natural Sciences and Engineering Research Council of Canada (NSERC) - Conseil de recherche en sciences naturelles et en génie du Canada (CRSNG)innovation.caHuman Frontier Science Program