Dissociated Hippocampal Cultures

TitleDissociated Hippocampal Cultures
Publication TypeBook Chapter
Year of Publication2010
AuthorsNault F, De Koninck P
EditorDoering LC
Book TitleProtocols for Neural Cell Culture: Fourth Edition
Pagination137 - 159
PublisherHumana Press
CityTotowa, NJ
ISBN Number978-1-60761-292-6

A popular approach to study living neurons involves the preparation of dissociated cultures. If isolated from neonatal or embryonic animals, neurons survive their removal and subsequent dissociation procedures. They grow processes on appropriate substrates, acquire mature neuronal morphology and polarity, and form functional synapses that resemble those of neurons developing in vivo. On the other hand, dissociated neurons produce neural circuits which differ from native circuits.



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