Nanoparticle-Assisted Localized Optical Stimulation of Cultured Neurons

TitleNanoparticle-Assisted Localized Optical Stimulation of Cultured Neurons
Publication TypeBook Chapter
Year of Publication2018
AuthorsLavoie-Cardinal F, Salesse C, Ayotte-Nadeau P-L, De Koninck P
EditorSantamaria F, Peralta XG
Book TitleUse of Nanoparticles in Neuroscience
Pagination15 - 23
PublisherSpringer New York
CityNew York, NY
ISBN Number978-1-4939-7584-6

Nanoparticle-assisted localized optical stimulation (NALOS) of cultured neurons is an all-optical method that allows subcellular light stimulation to investigate localized signaling in neurons. The stimulation and monitoring of localized Ca2+ signaling in neurons takes advantage of plasmonic excitation of gold nanoparticles (AuNPs) with infrared light. In this chapter we describe how NALOS, through its effects localized to region smaller than 10 μm2, may be a useful complement to other light-dependent methods for controlling neuronal activity and cell signaling. We demonstrate that this technique can be applied to cultured hippocampal neurons using commercially available bare AuNPs.



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Canadian Institutes of Health Research - Instituts de recherche en santé du Canada Fonds de recherche du Québec – Nature et technologies (FRQNT)Fonds de la recherche en santé du Québec   Natural Sciences and Engineering Research Council of Canada (NSERC) - Conseil de recherche en sciences naturelles et en génie du Canada (CRSNG)innovation.caHuman Frontier Science ProgramCanada First Research Excellence FundSentinelle Nord