The dynamic interplay between ATP/ADP levels and autophagy sustain neuronal migration in vivo

TitleThe dynamic interplay between ATP/ADP levels and autophagy sustain neuronal migration in vivo
Publication TypeJournal Article
Year of Publication2020
AuthorsBressan C, Pecora A, Gagnon D, Snapyan M, Labrecque S, De Koninck P, Parent M, Saghatelyan A
Secondary AuthorsD Abrous N
JournaleLife
Volume9
Paginatione56006
Date Published2020/09/28
ISBN Number2050-084X
Abstract

Cell migration is a dynamic process that entails extensive protein synthesis and recycling, structural remodeling, and considerable bioenergetic demand. Autophagy is one of the pathways that maintain cellular homeostasis. Time-lapse imaging of autophagosomes and ATP/ADP levels in migrating cells in the rostral migratory stream of mice revealed that decreases in ATP levels force cells into the stationary phase and induce autophagy. Pharmacological or genetic impairments of autophagy in neuroblasts using either bafilomycin, inducible conditional mice, or CRISPR/Cas9 gene editing decreased cell migration due to the longer duration of the stationary phase. Autophagy is modulated in response to migration-promoting and inhibiting molecular cues and is required for the recycling of focal adhesions. Our results show that autophagy and energy consumption act in concert in migrating cells to dynamically regulate the pace and periodicity of the migratory and stationary phases in order to sustain neuronal migration.

URLhttps://doi.org/10.7554/eLife.56006

Funding

Our research endeavors are made possible by the following agencies:

Canadian Institutes of Health Research - Instituts de recherche en santé du Canada Fonds de recherche du Québec – Nature et technologies (FRQNT)Fonds de la recherche en santé du Québec  Natural Sciences and Engineering Research Council of Canada (NSERC) - Conseil de recherche en sciences naturelles et en génie du Canada (CRSNG)innovation.caHuman Frontier Science ProgramCanada First Research Excellence FundSentinelle Nord